adult primary human dermal lymphatic endothelial cells hdlecs  (PromoCell)

Journal: bioRxiv

Article Title: Lymphatic Endothelial Cells Regulate Neutrophil Phenotypes and Function in a Microphysiological Model of Infection

doi: 10.64898/2026.03.24.714048

Figure Lengend Snippet: (A) PDMS devices fabricated from soft lithography molds assembled onto MatTek glass-bottom culture dishes with integrated removable PDMS rods, and resultant lumen channels following rod removal after collagen polymerization. (B) Schematic showing the lumen seeding arrangement with one lumen seeded with human dermal lymphatic endothelial cells (HdLECs, yellow) to form a biomimetic lymphatic vessel and the adjacent lumen loaded with neutrophils (purple). pHrodo-conjugated bacterial bioparticles (green) are embedded within the surrounding collagen matrix. (C) Representative fluorescence image of a biomimetic lymphatic vessel formed within the collagen matrix.

Article Snippet: Adult primary human dermal lymphatic endothelial cells (HdLECs) were purchased from PromoCell (C-12217).

Techniques: Fluorescence

Journal: Advanced Healthcare Materials

Article Title: A Human‐Based Skin‐Lymphoreticular Model‐on‐Chip to Emulate Inflammatory Skin Conditions

doi: 10.1002/adhm.202503170

Figure Lengend Snippet: Integration of lymphatic endothelial cells (LEC) increases the biomimicry of a self‐assembled lymphoreticular (LR) model. A) Exemplary histological staining of a human lymph node. Scale bar = 1 mm. Copyright permission has been obtained from Xinxiang Happy Science Co., Ltd. B) Schematic illustration of the layering approach. C) Brightfield image of a self‐assembled LR model. D) Representative H&E staining and E) IF images showing the FRC‐derived ECM marker ER‐TR7 (red), DAPI (blue) and the localization of GFP‐tagged LECs of LR models generated using different layering approaches: FLF (FRCs + GPF‐LECs + FRC), FLFN (FRC + GPF‐LECs + (FRC + naïve CD4 + T cells)), FLFA (FRC + GPF‐LECs + (FRC + activated CD4 + T cells)), FLN (FRC + GPF‐LECs + naïve CD4 + T cells), and FLA (FRC + GPF‐LECs + activated CD4 + T cells). Circles and arrows indicate the compartmentalization within LR models. Semi‐quantification of F) ER‐TR7 expression and G) GFP‐LEC distribution in the LR models generated using the different layering approaches. n = 3. One‐way ANOVA followed with Tukey post‐hoc tests were performed. Mean ± SD. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, ****p ≤ 0.0001. H) IF staining against collagen type 1 and fibronectin as key ECM components of lymphoid tissue in FLFA models. Cell nuclei are counterstained with DAPI (blue). Scale bars = 100 µm.

Article Snippet: Green fluorescent protein‐expressing dermal primary human lymphatic endothelial cells (GFP – LECs) (cAP‐0003GFP, male donor) and the Quick Coating Solution (cAP‐01) were purchased from Angio‐Proteomie.

Techniques: Staining, Derivative Assay, Marker, Generated, Expressing